Lignin Decolorization and Degradation of Pulp and Paper Mill Effluent by Ligninolytic Bacteria


1 Environmental Health & Safety Department, Leayan Global Pvt. Ltd., Kanpur- 208012, India

2 Department of Civil Engineering, Institute of Engineering & Technology Lucknow-226021, India

3 Environmental Monitoring Laboratory, Environmental Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), M.G. Marg, Lucknow-226001, India


The aim of this research work is to isolate bacterial strains with high potential in the degradation and decolorization of lignocellulose compounds of paper mill effluent. Four bacterial strains were isolated from marine sediments and they were screened to their ability to degrade the lignin and decolorize the Century pulp and paper mill effluent. Among four bacterial strains, three bacterial strains Bacillus subtilis, Bacillus endo-phyticus, Bacillus sp. were capable of ligninolytic activity. Consortium made by these bacterial strains enhances the degradation of lignin as well as decolorization. Various nitrogen source, carbon source, pH, temperature and low molecular weight organic acids were used in the optimization process of decolorization and degradation of lignin in paper mill effluent. Maximum decolorization 68.29% was found at pH 7.92, temperature 33°C, in the presence of glucose (as carbon source) 0.99% and yeast extract (as nitrogen source) 0.36% when it was optimized through response surface methodology.


  1. Ebrahiem, E.E., Al-Maghrabi, M.N., Mobarki, A.R., 2013. Removal of organic pollutants from industrial wastewater by applying photo-Fenton oxidation technology, Arabian Journal of Chemistry, doi:10.1016/j.arabjc.2013.06.012.
  2. Kesalkar, V.P., Khedikar, I.P., Sudame, A.M., 2012. Physico-chemical characteristics of wastewater from Paper Industry. International Journal of Engineering Research, 2(4), 137-143.
  3. Haider, K., Trojanaski, J., 1980. A comparasion of the degradation of 14C-labelled DHP and corn stalk lignin by macro fungi and bacteria. In: Kirk T.K., Higuchi, T., Chang, H-M. (eds.) Lignin biodegradation: Microbiology, Chemistry and Application, 1, CRC press, USA, 111-134.
  4. Daniel, G., Nilsson, T. 1998. Development in the study of soft rot and bacterial decay. In:Bruce.A., Palfreyman J.W. (eds.) Forest Product Biotechnology. Taylor & Francis, Great Britain, 37-62.
  5. Benner, R., Newell, S.Y., Maccrubbin, A.E., Hodson, R.E. 1984. Anaerobic degradation of lignin and polysaccharide component of lignocellulose and synthetic lignin by sediment microflora. Applied and environmental microbiology. 47(5), 998-1004.
  6. Odier, E., Jain, G., Monties, B., 1981. Poplar lignin decomposition by gram-negative aerobic bacteria. Applied and Environmental Microbiology, 41,  337-341.
  7. Gonzalez, J.M., Mayer, F.M., M.A., Hodson, R.E., Whitman, W.B., 1997, Sagittula stellata gen. nov., sp. nov., a Lignin-Transforming Bacterium from a Coastal Environment, International journal of systematic bacteriology, 773-780.
  8. Bourbonnais, R., Paice, M.G. 1990. Oxidation of non-phenolic substrates. An expanded role for laccase in lignin biodegradation. FEBS Letters, 267, 99-102.
  9. Reyes, P., Pickard, M.A., Vazquez-Duhalt, R., 1999. Hydroxybenzotriazole increases the range of textile dyes decolorized by immobilized laccase. Biotechnology Letters, 21, 875-880.
  10. Myers, R.H., Montgomery, D.C., 2002. Response Surface Methodology: Process and Product Optimization Using Designed Experiments. 2nd ed., Wiley, New York.
  11. Box, G.E.P., Draper, N.R. 1987. Empirical Model-Building and Response Surfaces. John Wiley & Sons, New York.
  12. Toropov, V.V., Van-Keulen, F., Markine, V.L., de-Boer, H., 1996. Refinements in the multi-point approximation method to reduce the effects of noisy responses, 6th AIAA/USAF/NASA/ISSMO Symposium on Multidisciplinary Analysis and Optimization, Bellevue WA, Part 2, 941-951.
  13. C.P.P.A. 1974. Technical section standard method H5P. Montreal, Canada: Canadian Pulp and Paper Association.
  14. Ulmer, D., Leisola, M., Schmidt, B., Fiechter, A., 1983. Rapid degradation of isolated lignins by Phanerochaete chrysosporium. Applied and Environmental Microbiology, 45, 1795-1801.
  15. Perestelo, F., Falcon, M.A., Perez, M.L., Roig, E.C., de, la, Fuente, Martin, G., 1989. Bioalteration of Kraft Pine Lignin by Bacillus rnegaterium Isolated from Compost Piles, Journal of Fermentation Bioengineering, 68(2), 151-153.
  16. Donderski, W., Mudryk, Z., Walczak, M., 1998. Utilization of Low Molecular Weight Organic Compounds by Marine Neustonic and Planktonic Bacteria. Polish Journal of Environmental Studies, 7(5), 279-283.
  17. Montgomery, Douglas, C., 2005. Design and Analysis of Experiments Response Surface Method and Designs (New Jersey, John Wiley and Sons, Inc).
  18. Oehlert, G.W., 2000. Design and Analysis of Experiments: Response Surface Design (New York: W.H. Freeman and Company).
  19. Myers, R.H., Montgomery, D.C., 2001. Response surface methodology, 2nd edn. Wiley, New York.
  20. Nazzal, S., Khan, M.A., 2002. Response surface methodology for optimization of ubiquinone self-nanoemulsified drug delivery system, AAPS PharmSciTech. 3, 1-9.
  21. Esbensen, K.H., 2005. Multivariate data analysis-in practice, CAMO Software AS, 5th edn, Oslo, Norway.
  22. Desai, K.M., Survase, S.S., Saudagar, P.S., Lele, S.S., Singhal, R.S. 2008. Comparison of artificial neural network (ANN) and response surface methodology (RSM) in fermentation media optimization: case study of fermentative production of scleroglucan. Biochemical Engineering Journal, 41(3), 266-273.
  23. Yetilmezsoy, K., Demirel, S., Vanderbei, R.J., 2009. Response surface modeling of pb(II) removal from aqueous solution by Pistaciavera L.: Box–Behnken experimental design, Journal of Hazardous Materials, 171, 551–562.
  24. Liu, H.L., Chiou, Y.R., 2005. Optimal decolorization efficiency of Reactive Red 239 by UV/TiO2 photocatalytic process coupled with response surface methodology, Chemical Engineering Journal, 112, 173-179.