Document Type : Original Article

Authors

Department of Environment, Abadeh Branch, Islamic Azad University, Abadeh, Iran

Abstract

In the wildlife management, maintaining water quality and quantity, especially in areas that are faced with relative constraints of water resouces, are considered as one of the planning pillars. Natural springs and artificial troughs in the KooheHava and TangeKhoor Free Area are the only sources of water suppliers for wildlife of the area. The aim of this study was to investigate the microbial indices of water resources used by wildlife in this areas and to compare them with the Iranian national standard limit. In this study, 12 water sources including ten springs and two troughs were selected and sampling was carried out in two seasons of summer and autumn of 2020 and three samples from each water resources and a total of 72 samples were collected throughout the study period and the parameters of total coliform, fecal coliform, temperature, turbidity and pH were measured. The data were analyzed by One sample t-test, Paired sample t-test, Independent sample t-test, analysis of variance and Spearman correlation matrix. The results showed that the means of total coliform were higher than the standard limit in all samples with the exception of no. 4 and 12 springs and the means of fecal coliform were higher than the standard limit in all samples with the exception of no. 2, 4, 8, 10, 11 and 12 water resources both during summer and autumn. In summer, with increasing evaporation, the amount of pollution load of water resources was higher than autumn. However in autumn, the number of polluted water resources was higher which was due to the transmission of microbial contaminations caused by human and animal feces via rain. The results of correlation showed a decrease or an increase in turbidity, temperature or pH did not affect the amount of coliforms because despite the strong correlation between total coliform and fecal coliform, no correlation was found between them and physicochemical factors of water. Therefore, considering the temporal and spatial variability of fecal coliforms and their effect on disease, death and reduction of wildlife populations, optimizing and disinfection of water resources with chlorine and dredging them are recommended.

Keywords

Main Subjects

  1. Zemanova, M.A., 2020. Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods. Wildlife Biology, 2020(1), pp.1–17. Doi: 10.2981/wlb.00607
  2. Small, J., 2021. The Wildlife Techniques Manual. Human-Wildlife Interactions, 15(1), pp.1–2.
  3. Ajani, O.O., Olutona, G.O., and Adeniji, A.A., 2021. Empirical Study of the Groundwater Protection Potential and Water Quality Using the Electrical Resistivity Method and a PG990 Spectrometer Around Obafemi Awolowo University Dumpsite Southwestern Nigeria. Iranian Journal of Energy and Environment, 12(4), pp.358–366. Doi: 10.5829/IJEE.2021.12.04.09
  4. Khadam, I.M., and Kaluarachchi, J.J., 2006. Water quality modeling under hydrologic variability and parameter uncertainty using erosion-scaled export coefficients. Journal of Hydrology, 330(1–2), pp.354–367. Doi: 10.1016/j.jhydrol.2006.03.033
  5. Zare Bidoki, G., Oladi, J., Fallah, A., and Sotoudeh, A., 2022. Development of urban forestry with the help of GIS and RS (study area: Yazd city). In: The 2nd International conference on planning agricultural sciences, environment, geography and sustainable energy. Mashhad.
  6. McArthur, J.M., Sikdar, P.K., Hoque, M.A., and Ghosal, U., 2012. Waste-water impacts on groundwater: Cl/Br ratios and implications for arsenic pollution of groundwater in the Bengal Basin and Red River Basin, Vietnam. Science of The Total Environment, 437, pp.390–402. Doi: 10.1016/j.scitotenv.2012.07.068
  7. Yousefi, Z., Ala, A., and Eslamifar, M., 2018. Evaluation of the presence of coliform in bottled drinking water, released in Sari in 2016. Environmental Health Engineering and Management, 5(3), pp.181–186. Doi: 10.15171/EHEM.2018.25
  8. Von Gunten, U., 2003. Ozonation of drinking water: Part II. Disinfection and by-product formation in presence of bromide, iodide or chlorine. Water Research, 37(7), pp.1469–1487. Doi: 10.1016/S0043-1354(02)00458-X
  9. Pandey, P.K., Kass, P.H., Soupir, M.L., Biswas, S., and Singh, V.P., 2014. Contamination of water resources by pathogenic bacteria. Amb Express, 4, pp.1–16.
  10. Bitton, G., 2005. Wastewater microbiology. John Wiley & Sons.
  11. Tully, T.N., and Shane, S.M., 1997. Ratite management, medicine and surgery. Journal of the South African Veterinary Association, 68(2), pp.54.
  12. ASHBOLT, R., and KIRK, M.D., 2006. Salmonella Mississippi infections in Tasmania: the role of native Australian animals and untreated drinking water. Epidemiology and Infection, 134(6), pp.1257–1265. Doi: 10.1017/S0950268806006224
  13. Bruslind, L., 2020. General microbiology. Oregon State University.
  14. Sastry, A.S., and Bhat, S., 2018. Essentials of medical microbiology. JP Medical Ltd.
  15. Blanco, G., Lemus, J.A., and Grande, J., 2009. RETRACTED: Microbial pollution in wildlife: Linking agricultural manuring and bacterial antibiotic resistance in red-billed choughs. Environmental Research, 109(4), pp.405–412. Doi: 10.1016/j.envres.2009.01.007
  16. Varunprasath, K., and A Daniel, N., 2010. Physico-chemical parameters of river Bhavani in three stations, Tamilnadu, India. Iranian Journal of Energy & Environment, 1(4), pp.321–325.
  17. Pishva, E., 2011. Occurrence of Escherichia coli in wild Guinea pigs fecal pellets from Karkas Mountain, Iran. Journal of Health System Research, 6(1), pp.37–43. [In Persian]
  18. Masoudi, M., Davoodian, A., Pajoohesh, M., Mircholi, F., Mirsoleimani, M., and Keihanpanah, M., 2015. Evaluation of microbial pollution of drinking water in North-West Eghlid. Journal of Sabzevar University of Medical Sciences, 22(2), pp.516–522. [In Persian]
  19. Faeeid, M., Babaei, H., and Abedini, A., 2015. Microbial and physicochemical parameters of the Anzali wetland. Journal of Wetland Ecobiology, 7(3), pp.45–54. [In Persian]
  20. Aligholizadeh, F., Asgharnia, H.A., Amouei, A., and Zabihzadehe Pasha, H., 2020. Investigation of microbial water quality of springs in the mobile areas of the eastern gorge of Babol city in 2018. In: 20th Annual Research Congress of Medical Students. Kermanshah, Iran. [In Persian]
  21. Haghighat, R., and Nowzari, H., 2021. Study of the Beshar River water quality in the Dena Protected Area. Journal of Wetland Ecobiology, 13(2), pp.69–84. [In Persian]
  22. Cox, P., Griffith, M., Angles, M., Deere, D., and Ferguson, C., 2005. Concentrations of Pathogens and Indicators in Animal Feces in the Sydney Watershed. Applied and Environmental Microbiology, 71(10), pp.5929–5934. Doi: 10.1128/AEM.71.10.5929-5934.2005
  23. Ghimire, N.P., Caravellol, G., and Jha, P.K., 2013. Bacterial Contamination in the Surface Waterbodies in Sagarmatha National Park and Buffer Zone, Nepal. Scientific World, 11(11), pp.94–96. Doi: 10.3126/sw.v11i11.8560
  24. Divya, A.H., and Solomon, P.A., 2016. Effects of Some Water Quality Parameters Especially Total Coliform and Fecal Coliform in Surface Water of Chalakudy River. Procedia Technology, 24, pp.631–638. Doi: 10.1016/j.protcy.2016.05.151
  25. Reed, B.C., and Rasnake, M.S., 2016. An Assessment of Coliform Bacteria in Water Sources Near Appalachian Trail Shelters Within the Great Smoky Mountains National Park. Wilderness & Environmental Medicine, 27(1), pp.107–110. Doi: 10.1016/j.wem.2015.09.019
  26. Bojarczuk, A., Jelonkiewicz, Ł., and Lenart-Boroń, A., 2018. The effect of anthropogenic and natural factors on the prevalence of physicochemical parameters of water and bacterial water quality indicators along the river Białka, southern Poland. Environmental Science and Pollution Research, 25(10), pp.10102–10114. Doi: 10.1007/s11356-018-1212-2
  27. Ogwueleka, T.C., and Christopher, I.E., 2020. Hydrochemical interfaces and spatial assessment of Usuma River water quality in North-Central Nigeria. Scientific African, 8, pp.e00371. Doi: 10.1016/j.sciaf.2020.e00371
  28. Fars Department of Environment Portal, 2022. No-hunting Area. https://Fars.Doe.ir/portal/home
  29. Faraji, E., 1999. Weather and Climatology. Tehran, Publ Carno.
  30. World Health Organization, 2017. Guidelines for drinking-water quality: 4th edition, 631 p. ISBN 978-92-4-154995-0
  31. American Public Health Association (APHA), 2005. Standard methods for the examination of water and wastewater. 20th edition, Washington D.C.
  32. SPSS Inc. Released, 2008. SPSS Statistics for windows. Version 17.0. Chicago. IL: SPSS Inc.
  33. Iranian national standard, 2019. Drinking water- Microbiological specification. ISIRI number 1011, Institute of Standards and Industrial Research of Iran, Seventh edition.
  34. Carson, C.A., Shear, B.L., Ellersieck, M.R., and Asfaw, A., 2001. Identification of Fecal Escherichia coli from Humans and Animals by Ribotyping. Applied and Environmental Microbiology, 67(4), pp.1503–1507. Doi: 10.1128/AEM.67.4.1503-1507.2001
  35. Buckalew, D.W., Hartman, L.J., Grimsley, G.A., Martin, A.E., and Register, K.M., 2006. A long-term study comparing membrane filtration with Colilert® defined substrates in detecting fecal coliforms and Escherichia coli in natural waters. Journal of Environmental Management, 80(3), pp.191–197. Doi: 10.1016/j.jenvman.2005.08.024
  36. Wei, X., Li, J., Hou, S., Xu, C., Zhang, H., Atwill, E., Li, X., Yang, Z., and Chen, S., 2018. Assessment of Microbiological Safety of Water in Public Swimming Pools in Guangzhou, China. International Journal of Environmental Research and Public Health, 15(7), pp.1416. Doi: 10.3390/ijerph15071416
  37. Hackbarth, C., and Weissinger, R., 2018. Escherichia coli concentrations in Fremont River tributaries at Capitol Reef National Park, 2008–2017.
  38. Tulagi, A., 2017. Waikato river water quality monitoring programme: Data Report 2017. TR 2018/24, Waikato Regional Council Technical, Hamilton.
  39. Bai, V.R., Kit, A.C., Kangadharan, G., Gopinath, R., Varadarajan, P., and Hao, A.J., 2022. Experimental study on total coliform violations in the complied NH2CL, O3, and UV treated municipal water supply system. The European Physical Journal Plus, 137(6), pp.689. Doi: 10.1140/epjp/s13360-022-02891-5
  40. Pandey, P., Soupir, M.L., Wang, Y., Cao, W., Biswas, S., Vaddella, V., Atwill, R., Merwade, V., and Pasternack, G., 2018. Water and Sediment Microbial Quality of Mountain and Agricultural Streams. Journal of Environmental Quality, 47(5), pp.985–996. Doi: 10.2134/jeq2017.12.0483
  41. Zhang, X., Li, Y., Liu, B., Wang, J., Feng, C., Gao, M., and Wang, L., 2014. Prevalence of Veterinary Antibiotics and Antibiotic-Resistant Escherichia coli in the Surface Water of a Livestock Production Region in Northern China. PLoS ONE, 9(11), pp.e111026. Doi: 10.1371/journal.pone.0111026