Effect of Substrate and Granules/Inocula Sizes on Biochemical Methane Potential and Methane Kinetics


1 School of Engineering and Resources, Walailak University, Nakhon Si Thammarat, Thailand

2 Department of Biology, Faculty of Science, Thaksin University, Patthalung, Thailand

3 School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia


This study aimed  to  evaluate  the  Biochemical Methane Potential (BMP) of different types of wastewaters and sizes of granules. The granules (CS: from a cassava, SS: a seafood, and PS: a palm oil factory) and wastewaters initial Chemical Oxygen Demand (COD) were 18,800, 4,200 and 100,000 mg/l  respectively).  Modified  Gompertz  equation  was  used  to compare the data from the experiments. Wastewater from a cassava factory gave the highest BMP when used with only granules from its own source (CS). Wastewater from seafood factory had the highest nitrogen content thus, represented the most imbalance nutrient source. In this case, mix- granules (SS+CS) gave highest BMP. Palm oil mill effluent did not match COD: N ratio criterion and had too high COD level which caused substrate inhibition. Here the mix-granules (PS+CS) gave highest  BMP.  In  general,  the  larger  granule  size  and  the  nutrient  balance  could  improve  the efficiency and hence increase the biogas production rate. The initial COD or different substrate has a strong effect on BMP and the maximum specific methane rates whereas the different sizes of granule have an effect on the length of lag phase period. In most cases, it was sufficient to represent the experimental data with traditional modified Gompertz equation and Monod models.


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