Authors
1 Mineral Industries Research Center, Shahid Bahonar University of Kerman, Iran
2 Department of Chemical and Polymer Engineering, Faculty of Engineering, Yazd University, Yazd, Iran
Abstract
This research work demonstrates the feasibility of accelerating bioremediation of a clay soil by supplementing with (NH4)2SO4, KH2PO4, sucrose (as an inducer for growth) and tween 80. The soil contained 7% residual gasoil. The bioremediation was stimulated by moisture adjustment to 10%, and inoculating with hydrocarbon degrading microorganisms. (NH4)2SO4 and KH2PO4 were added to the soil to obtain soil samples with C:N:P ratios of 100:1.4:1.4, 100:6.4:1.9, 100:11.4:2.4, and 100:21.4:3.4. The C:N:P of 100:11.4:2.4 resulted in more than 78% gasoil removal for duration of two months. Tween 80, in the range of 0-20mL/ (kg soil), was added to the soil samples with C:N:P ratio of 100:1.4:1.4. More than 84% removal was achieved when 10 mL/kg tween 80 was added to the soil. Sucrose, in the range of 0-20 g/(kg soil), was added to the soil samples with the C:N:P ratio of 100:1.4:1.4. For the sucrose level of 20 g/(kg soil), 79% removal was obtained in two months. Additional experiment was also conducted at two porosity levels of 54% and 22%. The removal percentage in the soil with high porosity was almost twice as compared to soil with low porosity.
Keywords
- Abedi-Koupai, J., R. Ezzatian, M. Vossoughi-Shavari, S. Yaghmaei and M. Borghei, 2007. The effects of microbial population on phytoremediation of petroleum contaminated soils using tall fescue. International Journal of Agricultural Biology, 9(2): 242–246
- Carman, K.R., J.W. Fleeger and S.M. Pomarico, 2000. Does historical exposure to hydrocarbon contamination alter the response of benthic communities to diesel contamination? Marine Environmental Research, 49(2):255–27.
- Pritchard, P.H., J.G. Mueller, J.C. Rogers,F.V. Kremer and J.A. Glaser,1992. Oil spill bioremediation: experiences, lessons and results from the Exxon Valdez oil spill in Alaska. Biodegradation, 3: 315-335.
- Nikolopoulou, M. and N. Kalogerakis, 2008. Enhanced bioremediation of crude oil utilizing lipophilic fertilizers combined with biosurfactants and molasses. Marine Pollution Bulletin, 56:1855–1861.
- Laha, S., B. Tansel and A. Ussawarujikulchai, 2009. Surfactant–soil interactions during surfactant amended remediation of contaminated soils by hydrophobic organic compounds: a review. Journal of Environmental Management, 90(1): 95–100.
- Tiehm, A., M. Stieber, P. Werner and F.H. Frimmel, 1997. Surfactant-enhanced mobilization and biodegradation of polycyclic aromatic hydrocarbons in manufactured gas plant soil. Environmental Science and Technology, 31(9): 2570–2576.
- Makkar, R. and K. Rockne, 2003. Comparison of synthetic surfactants and biosurfactant in enhancing biodegradation of polycyclic aromatic hydrocarbons. Environmental Toxicology and Chemistry, 22(10): 2280-2292.
- Kim, H.S. and W.J. Weber, 2005. Polycyclic aromatic hydrocarbon behavior in bioactive soil slurry reactors amended with a nonionic surfactant. Environmental Toxicology and Chemistry, 24(2): 268-276.
- Makkar, R.S. and K.J. Rockne, 2003. Comparison of synthetic surfactants and biosurfactant in enhancing biodegradation of polycyclic aromatic hydrocarbons. Environmental. Toxicology and Chemistry, 22(10):2280-2292.
- Obuekwe, C., Z.K. Al-Jadi and E. Al-Saleh, 2009. Hydrocarbon degradation in relation to cell-surface hydrophobicity among bacterial hydrocarbon degraders from petroleum-contaminated Kuwait desert environment. International Biodeterioration and Biodegradation, 63(3): 273–279
- Paria, S.2008. Surfactant-enhanced remediation of organic contaminated soil and water. Advances in Colloid and Interface Science, 138(1): 24–58.
- Urum, K., and T. Pekdemir, 2004. Evaluation of biosurfactant for crude oil contaminated soil washing. Chemosphere, 57(9)1139-1150.
- Yu, H., L. Zhu and W. Zhou, 2007. Enhanced desorption and biodegradation of phenanthrene in soil-water systems with the presence of anionic-nonionic mixed surfactants. Journal of Hazardous Materials, 142(2): 354-361.
- Akbari, A. and G. Subhasis, 2015. Bioaccessible porosity in Soil aggregates and implications for biodegradation of high molecular weight petroleum compounds. Environmental Science and Technology, 49(24): 14368−14375.
- Haghollahi, A., M.H. Fazaelipoor and M. Schaffie, 2016. The effect of soil type on the bioremediation of petroleum contaminated soils. Journal of Environmental Management, 180:197–201.
- Lee, S.H., S. Lee, D.Y. Kim and J.G. Kim, 2007. Degradation characteristics of waste lubricants under different nutrient condition. Journal of Hazardous Materials, 143: 65-72.
- Hesnawi, R.M. and M.M. Adbeib, 2013. Effect of nutrient source on indigenous biodegradation of diesel fuel contaminated soil. APCBEE Procedia, 5:557-561.
- Singh, R.P., G. Dhania, A. Sharma and P.K. Jaiwal, 2007. Biotechnological approaches to improve phytoremediation efficiency for environment contaminants. Environmental bioremediation technologies. Springer.
- Kim, I.S., J.S. Park and K.W Kim, 2001. Enhanced biodegradation of polycyclic aromatic hydrocarbons using non-ionic surfactants in soil slurry. Appllied. Geochemistry, 16:1419-1428.
- Sihag, S., H. Pathak and D.P. Jaroli, 2014. Factors affecting the rate of biodegradation of polyaromatic hydrocarbons. International journal of Pure and Applied Bioscience, 2:185-202